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Total No. of Questions : 8
[Total No. of Printed Pages : 2
[2]
Roll No t
MPH-101T/MIP-101T
M.Pharmacy (PCI), I Semester
Examination, June 2025
Modern Pharmaceutical Analytical Techniques
Time : Three Hours
Maximum Marks : 70
Note: i) Attempt any five questions.
ii) All questions carry equal marks.
1. a)
Discuss the significance of chromophores in UV-visible spectroscopy and explain how they affect absorption spectra.
b)
Explain the principle of Radioimmunoassay (RIA) and describe how it is used in diagnostic testing.
2. a)
Explain the working principle of a dispersive IR spectrometer and describe its key components.
b)
Describe the basic principle of NMR spectroscopy and explain how it detects nuclei in a magnetic field.
3. a)
Explain the role of quenchers in fluorescence and describe how quenching is used to study molecular interactions.
b)
Describe the role of Fast Atom Bombardment (FAB) in mass spectrometry and explain its applications.
4. a)
Describe the role of hollow cathode lamps in atomic absorption spectroscopy.
b)
Discuss the importance of the mobile and stationary phases in chromatography and how they affect separation.
5. a)
Describe the technique of gel electrophoresis and its applications in DNA and protein analysis.
b)
Explain the role of FTIR spectroscopy in studying complex biological molecules and provide examples of applications.
6. a)
Describe the setup of an X-ray powder diffraction apparatus and explain its applications.
b)
Explain how bioluminescence assays work and describe their applications in molecular biology.
7. a)
Explain how mass fragmentation patterns are used to determine molecular structure.
b)
Discuss the use of isoelectric focusing on protein purification and characterization.
8. a)
Describe the factors that affect retention time in gas chromatography and explain how they impact the analysis.
b)
Explain the concept of coupling constant and discuss how it is used to interpret NMR spectra.